The molecular biology core includes PCR machines, Real Time PCRs, droplet digital PCR, Centrifuges, water baths, sonicators, lyophilizers, MilliQ water system, Nanodrop, spectrophotometers. Transfer apparatus, Gel documentation, fume hoods are also part of the facility. 2D Electrophoresis for protein profiling is also a part of the facility.
Molecular Biology Core:
Cloning and Expression facility:
Antigen design, PCR machines, Bacterial shaker incubator, Biosafety Cabinets, DNA and Protein gel apparatus, CO2 shaker incubator for mammalian expression
In vitro Cell Culture facility:
Centre has two cell culture facilities including a primary culture as well
as a cell line facility. 2D drug resistant models, patient-derived cell lines and in vitro 3D models
biomimicking disease biology and drug resistance are the major models used/developed in the
Imaging facility/Flow Cytometry:
The imaging facility includes fluorescence microscopes, and the
flow cytometer (BD FACS Canto II).
Antibody Development Facility:
Immunization, booster and maintenance of responsive rodents
(through collaboration with In Vivo Biosciences), In house polyclonal antibody purification,
Monoclonal antibody generation (Fusion method) both hybridoma and recombinant, antibody
Conjugation of purified antibody and/or antibody fragments to
fluorophores or enzymes, ELISA (absorbance and fluorescence) assay development, validation and
Histology and Immunohistochemistry:
The centralized facility allows for preparation of FFPE blocks,
sectioning (microtome) and immunohistochemistry.
The biorepository is established in collaboration with MSMC, NH and archives samples from cancers of Head and Neck, Glioblastoma, Hematology, Breast cancers and is being expanded to other cancers. Open Specimen Software is used for repository management.
The Nanotechnology lab includes Dissolution Test Apparatus, Photometer 5010,
Electrospinning units, Sonicator, Lyophilizer, Diffusion cell apparatus and Cary 630 FTIR
MSMF has long standing collaborations with Medgenome, Eurofins and Institute of Bioinformatics (IOB) for work related to high throughput profiling including Transcriptomics, Proteomics and Genomics
In vivo model development
Animal studies are carried out in collaboration with multiple institutes
such as Syngene India, SRMC, Chennai. In vivo Biosciences, Bangalore