Tumor Immunology

Over the last two decades there has been a paradigm shift in our perception of cancer therapy. As we know now, neoplasia do not consist only of transformed cells, but contain an abundant and heterogeneous non-transformed component like stromal, endothelial and immune cells. The host immune system can recognize and sometimes react against (pre-) malignant cells as they transform, proliferate and evolve. The knowledge laid the grounds of anticancer immunosurveillance leading to cancer immunotherapy. Recent intense wave of preclinical and clinical investigation culminated in approval of various immunotherapeutic interventions for use in humans. In 2013, the extraordinary clinical success of immunotherapy has won this new method of therapy the title of “Breakthrough of the Year”. Nonetheless, we have just begun to unravel the therapeutic possibilities offered by anticancer immunotherapy.

The following programs have been envisioned to yield a number of Therapeutic and Diagnostic Products and processes

  • Development of Tumor Targeting Monoclonal Antibody Therapeutics
  • Development of Immunostimulants
  • Development of Immunomodulatory Monoclonal Antibody Therapeutics
  • Model Mammalian Cell line Development
  • Antibody library Generation

People

Manjula Das, PhD
Principal Investigator
manjula.das@ms-mf.org

PhD from Indian Institute of Science with training from McGil University – Canada and MIT-USA during post doctoral studies Manjula has spent long years in the Industry. She is an expert in Biotherapeutics especially critical reagent and assay development (in vitro and in vivo) for regulatory submission of protein drugs. She also has established many companies, the latest being a consultancy firm named Beyond Antibody. Manjula with 20-years long market experience concentrates on translating basic research to products and processes for direct patient care in various areas of cancer.

Current Lab

Christopher Bathula
Technical Assistant
bathulachristopher@ms-mf.org

 

Christopher, a project assistant, has developed expertise on tagged and untagged active protein purification from bacterial and mammalian sources.

                       C.A. Divya, MSc 
                        PhD Student
                  divya.ca@ms-mf.org

Divya is a PhD student with Mangalore University, is working on the role of inflammatory biomarkers in Diabetes and Cancer. She tests the therapeutic effect of Indian Ayurvedic medicines in bringing down the inflammatory conditions in in vitro and in vivo models.

               Mohit Srivastava, PG Biotech
                          PhD Student

Mohit, an ICMR PhD student with MAHE, is working towards understanding the role of Insulin receptor pathway in liquid malignancies and immunotherapy.

Nehanjali Dwivedi, MSc
PhD Student
nehanjali.dwivedi@ms-mf.org

Nehanjali is a PhD student with MAHE whose work primarily focuses on the role of LCN2, an inflammatory marker in oral cancer and Immunotherapy.


                       Smitha P.K
                     PhD Student
             smitha.pk@ms-mf.org

 

Smitha, a PhD student at Coimbatore University works on applications of various molecular biology techniques in plant and animal systems. The techniques include finding out reference genes by omic approach to developing recombinant monoclonal antibodies and developing novel fluorescent labelled immunoassays.

 

Sowmya T
SRF
sowmya.t@ms-mf.org

 

Sowmya, a SRF specialises in standardizing cytogenetics and cytotoxicity assay. She is working extensively on drug testing on primary bone marrow and PBMC culture and cell line establishment from patient samples with hematological malignancies.

Sreejeta Mondal
JRF
sreejeta.mondal@ms-mf.org

 

Sreejeta, after completing her MSc final year project has joined the lab as a JRF. She focuses on validating the anti-apoptotic marker BCL2 as a drug target with small molecules. She is developing a diagnostic platform for the same marker. She has the prime responsibility of maintaining the biorepository in the lab.

Vishnupriyan K, MSc
JRF
vishnupriyan@ms-mf.org

Vishnu, after completing his MSc final year project with Dr Manjula has joined the lab as a JRF. He focuses on honing his protein engineering skills to get higher affinity thera-diagnostic proteins and recombinant monoclonal antibodies in the area of cancer immunotherapy.

Dr. Narayana Subramanian

A PhD student under the special program of PhD for Practicing Clinicians, Dr Narayana Subramaniam is working on Immunotherapy in Oral Cancer. He is MBBS and MS in General Surgery from Ramaiah Medical college followed by MCh in Head and Neck Oncology from Amrita Institute of Medical Sciences, Kochi. He has also gained additional certification in transoral robotic surgery (TORS) from the University of Pennsylvania and robotic surgery from Delhi. Currently he is a Consultant and Assistant Fellowship Director, Head and Neck Oncology & Reconstructive Surgery, Mazumdar Shaw Medical Centre, Bangalore

Trainees

 

Alumni

Publications

  • Neutrophils homing into the retina trigger pathology in human patients and in a mouse model of early age-related macular degeneration Sayan Ghosh, Archana Padmanabhan, Tanuja Vaidya, Alan M. Watson, Imran A. Bhutto, Stacey Hose, Peng Shang, Nadezda Stepicheva, Meysam Yazdankhah, Joseph Weiss, Manjula Das, Santosh Gopikrishna, Aishwarya, Naresh Yadav, Thorsten Berger, Tak W. Mak, Shuli Xia, Jiang Qian, Gerard A. Lutty, Ashwath Jayagopal, J. Samuel Zigler, Jr., Swaminathan Sethu, James T. Handa, Simon C. Watkins, Arkasubhra Ghosh and Debasish Sinha; Nature Communications Biology , (Sep 2019): 2:348 : DOI 10.1038/s42003-019-0588-y
  • Genome Wide Search to Identify Reference Genes candidates for Gene Expression Analysis in Gossypium hirsutum Smitha P K, Vishnupriyan K , Ananya S.Kar, Anil Kumar M, Christopher Bathula, K.N. Chandrashekara, , Sujan K Dhar, Manjula Das : BMC Plant Biology; (2019) 19:405 https://doi.org/10.1186/s12870-019-1988-3
  • Engineered Antibodies: Sujan Dhar and Manjula Das: Journal of the Indian Institute of Science , Springer Verlag (March 2018): 98(1): 5-20; DOI: 10.1007/s41745-018-0061-9
  • Full-length cDNAs: more than just reaching the ends, Manjula Das, Isabel Harvey, Lee Lee Chu, Manisha Sinha and Jerry Pelletier. Physiological Genomics (2001) : 6 : 57-80; PMID: 11459922; DOI: 10. 1152/physiolgenomics.2001.6.2.57
  • Assessment of the total number of human transcription units, Manjula Das, Christopher Burge, Eunhee Park, Juliette Colinas, and Jerry Pelletier. Genomics (2001) : 77: 71-78; PMID: 11543635; DOI: 10.1006/geno.2001.6620
  • A Set of Canine Inter-repeat Sequence PCR Markers for High Throughput Genotyping, Manjula Das, Hakan Sakul, Julius Kong, Gregory Acland and Jerry Pelletier. Physiological Genomics (2000) : 4: 13-24; PMID: 11074009; DOI: 10.1152/physiolgenomics.2000.4.1.13
  • Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris, Manjula Das, Lee Lee Chu, Majuid Ghahremani, Tony Abramsgg, Michael Roy, David Housman, and Jerry Pelletier. Mammalian Genome (1998) : 9 : 64-69 PMID: 9434948
  • Comparative nucleotide and amino acid analysis of sequence-specific RNA-binding rotaviral nonstructural protein NSP3. CD.Rao, Manjula Das, P.Ilango, R.Lalwani, BS.Rao, K.Gowda, Virology (1995) : 207 : 327-333 PMID: 7871749; DOI: 10.1006/viro.1995.1087
  • Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (I321) have high levels of sequence identity with the homologous proteins of serotype 10 bovine rotavirus: Manjula Das, SJ.Dunn, GN.Woode, HB.Greenberg, CD.Rao Virology (1993): 194: 374-379. PMID: 8386881
  • Serotypic and Genotypic characterisation of human serotype 10 rotavirus from asymptomatic neonates SJ.Dunn, HB.Greenberg, RL.Ward, O.Nakagomi, JW.Burns, PT.Vo, KA.Pax, Manjula Das, K.Gowda, CD.Rao. J.Clin. Microbiology (1993): 31 : 135-169; PMID: 8380181; PMCID:PMC262646